Scientific Publications Resulting From ISLET Funding

Original research studies from ISLET partners made possible through ISLET funding:

Partner – Henrik Semb

Pancreatic alpha and beta cell fate choice is directed by apical-basal polarity dynamics

Tiemann U, Tian C, Hermann F, Proks M, Skovgaard E, Kulik I, Di Y, Sedzinski J, Semb H. Dev Cell. 2025 Mar 4:S1534-5807(25)00113-3. doi: 10.1016/j.devcel.2025.02.008. Epub ahead of print. PMID: 40056911.

Salt-inducible kinases transduce mechanical forces into the specification of the pancreatic endocrine lineage

Tian C, Rump A, Ebeid C, Mamidi A, Semb H. Stem Cell Reports. 2025 Apr 8;20(4):102444. doi: 10.1016/j.stemcr.2025.102444. Epub 2025 Mar 6. PMID: 40054471.

Partner – Heiko Lickert

Generation of pancreatic β cells from CD177⁺ anterior definitive endoderm.

Mahaddalkar PU, Scheibner K, Pfluger S, Ansarullah, Sterr M, Beckenbauer J, Irmler M, Beckers J, Knöbel S, Lickert H. Nat Biotechnol. 2020 Sep;38(9):1061-1072. doi: 10.1038/s41587-020-0492-5. Epub 2020 Apr 27. PMID: 32341565

Engineering islets from stem cells for advanced therapies of diabetes.

Siehler J, Blöchinger AK, Meier M, Lickert H. Nat Rev Drug Discov. 2021 Dec;20(12):920-940. doi: 10.1038/s41573-021-00262-w. Epub 2021 Aug 10. PMID: 34376833 Review.

Automated optimization of endoderm differentiation on chip.

Ardila Riveros JC, Blöchinger AK, Atwell S, Moussus M, Compera N, Rajabnia O, Georgiev T, Lickert H, Meier M. Lab Chip. 2021 Nov 25;21(23):4685-4695. doi: 10.1039/d1lc00565k.PMID: 34751293 Free PMC article.

Awaking sleeping islets for a cure of diabetes.

Scheibner K, Lickert H. Med. 2022 May 13;3(5):279-280. doi: 10.1016/j.medj.2022.04.004. Epub 2022 May 13. PMID: 35584645 No abstract available.

A transcriptional cross species map of pancreatic islet cells.

Tritschler S, Thomas M, Böttcher A, Ludwig B, Schmid J, Schubert U, Kemter E, Wolf E, Lickert H, Theis FJ. Mol Metab. 2022 Dec;66:101595. doi: 10.1016/j.molmet.2022.101595. Epub 2022 Sep 13. PMID: 36113773 Free PMC article.

Generation of ARX-T2A-H2B-CFP x C-PEP-mCherry-hiPSC double reporter line for monitoring of pancreatic differentiation.

Setyono ESA, Rogers NK, Hofmann A, Lickert H, Burtscher I.

Stem Cell Res. 2025 Apr;84:103685. doi: 10.1016/j.scr.2025.103685. Epub 2025 Feb 15.PMID: 40022904

Partner – Raphael Scharfmann

A 3D atlas of the human developing pancreas to explore progenitor proliferation and differentiation.

Villalba A, Gitton Y, Inoue M, Aiello V, Blain R, Toupin M, Mazaud-Guittot S, Rachdi L, Semb H, Chédotal A, Scharfmann R. Diabetologia. 2024 Jun;67(6):1066-1078. doi: 10.1007/s00125-024-06143-2. Epub 2024 Apr 17. PMID: 38630142 Free PMC article.

Imaging Human Pancreatic Endocrinogenesis During Early Prenatal Life.

Villalba A, Gitton Y, Aiello V, Toupin M, Mazaud-Guittot S, Chédotal A, Scharfmann R. Diabetes. 2025 Mar 1;74(3):368-375. doi: 10.2337/db24-0641. PMID: 39602451 Free PMC article.

Partner – Eelco De Koning

Stem cell islet replacement in type 1 diabetes: From “shelf” to “self”

De Koning EJP, Carlotti F. . Cell Stem Cell. 2024 Dec 5;31(12):1727-1729. doi: 10.1016/j.stem.2024.11.011. PMID: 39642862.

Partner – Matthias Mann

Proteomics Technology focused studies that advance methods for analysing cells:

Spatial single-cell mass spectrometry defines zonation of the hepatocyte proteome.

Rosenberger FA, Thielert M, Strauss MT, Schweizer L, Ammar C, Mädler SC, Metousis A, Skowronek P, Wahle M, Madden K, Gote-Schniering J, Semenova A, Schiller HB, Rodriguez E, Nordmann TM, Mund A, Mann M. Nat Methods. 2023 Oct;20(10):1530-1536. doi: 10.1038/s41592-023-02007-6. Epub 2023 Oct 2. PMID: 37783884; PMCID: PMC10555842.

Robust dimethyl-based multiplex-DIA doubles single-cell proteome depth via a reference channel

Thielert M, Itang EC, Ammar C, Rosenberger FA, Bludau I, Schweizer L, Nordmann TM, Skowronek P, Wahle M, Zeng WF, Zhou XX, Brunner AD, Richter S, Levesque MP, Theis FJ, Steger M, Mann M.  Mol Syst Biol. 2023 Sep 12;19(9):e11503. doi: 10.15252/msb.202211503. Epub 2023 Aug 21. PMID: 37602975; PMCID: PMC10495816.

Spatial proteo-transcriptomic profiling reveals the molecular landscape of borderline ovarian tumors and their invasive progression.

Schweizer L, Krishnan R, Shimizu A, Metousis A, Kenny H, Mendoza R, Nordmann TM, Rauch S, Kelliher L, Heide J, Rosenberger FA, Bilecz A, Borrego SN, Strauss MT, Thielert M, Rodriguez E, Müller-Reif JB, Chen M, Yamada SD, Mund A, Lastra RR, Mann M, Lengyel E.  medRxiv [Preprint]. 2023 Nov 13:2023.11.13.23298409. doi: 10.1101/2023.11.13.23298409. PMID: 38014221; PMCID: PMC10680885.

Synchro-PASEF Allows Precursor-Specific Fragment Ion Extraction and Interference Removal in Data-Independent Acquisition

Skowronek P, Krohs F, Lubeck M, Wallmann G, Itang ECM, Koval P, Wahle M, Thielert M, Meier F, Willems S, Raether O, Mann M. . Mol Cell Proteomics. 2023 Feb;22(2):100489. doi: 10.1016/j.mcpro.2022.100489. Epub 2022 Dec 22. PMID: 36566012; PMCID: PMC9868879.

IMBAS-MS Discovers Organ-Specific HLA Peptide Patterns in Plasma.

Wahle M, Thielert M, Zwiebel M, Skowronek P, Zeng WF, Mann M. Mol Cell Proteomics. 2024 Jan;23(1):100689. doi: 10.1016/j.mcpro.2023.100689. Epub 2023 Dec 1. PMID: 38043703; PMCID: PMC10765297.

Spatial Proteomics of Single Hepatocytes with Multiplexed Data-Independent Acquisition (mDIA).

Thielert M, Weiss CAM, Mann M, Rosenberger FA. Methods Mol Biol. 2024;2817:97-113. doi: 10.1007/978-1-0716-3934-4_9. PMID: 38907150.

An accessible workflow for high-sensitivity proteomics using parallel accumulation-serial fragmentation (PASEF).

Skowronek P, Wallmann G, Wahle M, Willems S, Mann M. Nat Protoc. 2025 Jan 17. doi: 10.1038/s41596-024-01104-w. Epub ahead of print. PMID: 39825144.